mouse anti pig siga  (Bio-Rad)

Journal: Frontiers in Immunology

Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs

doi: 10.3389/fimmu.2025.1606128

Figure Lengend Snippet: Strong positive correlation between sIgA against A . suum adult ES and eosinophil influx. (A) Pearson correlation of sIgA against adult ES products and %eosinophil frequencies of leukocytes in BAL . (B) Box plots illustrating median IgM, IgG and IgA responses against A . suum adult ES products in BAL fluid. (C) Box plots illustrating sIgA against L3 lysate, adult lysate and adult ES in BAL fluid. Blue and red denotes uninfected controls and A. suum infected pigs, respectively. Whiskers indicate 95% percentile. Significance determined by Wilcoxon test is represented by p<0.05: *p<0.01: **.

Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.

Techniques: Infection

Journal: Frontiers in Immunology

Article Title: Larval ascariasis elicits a prominent IgA and IgG1/2 antibody response to adult Ascaris excretory/secretory antigens in pigs

doi: 10.3389/fimmu.2025.1606128

Figure Lengend Snippet: IgA + B cells are highly induced in the ileal mLN 14 dpi. (A) Boxplots of sIgA against the three antigens in intestinal mucus. Exemplary flow cytometry plots depicting the gating strategy used to identify CD79a + B cell populations in mLNs. Representative flow cytometry plots of IgA + cells gated on CD79a + cells and bar plots of mean frequencies of % IgA producing B cells in jejunal (B) and ileal (C) mLN 14 dpi. (D) Representative flow cytometry plots of IgA + cells gated on CD79a + cells and bar plots of mean frequencies of % IgA producing B cells in jejunal mLN 35 dpi. Bar plot error bars indicate mean sd. Boxplot whiskers indicate 95% percentile. Significance determined by Wilcoxon test is represented by p<0.05: *p<0.01: **.

Article Snippet: For IgM, IgA, IgG1 and IgG2, non-conjugated mouse anti-pig IgM (Bio-Rad, # MCA637GA) diluted at 1:20000, mouse anti-pig IgA (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig IgG1 (Bio-Rad, # MCA635GA) diluted at 1:1000 and mouse anti-pig IgG2 (Bio-Rad, # MCA638GA) diluted at 1:1000, mouse anti-pig sIgA (Bio-Rad, # MCA634GA) diluted at 1:10000 was used.

Techniques: Flow Cytometry

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: The levels of SARS-CoV-2 anti-RBD (A) and NP (B) were measured in serum using Roche Elecsys anti-SARS-CoV-2 S (A) and SARS-CoV-2 (B) total Ig assay. (C) Levels of total IgA in the NLF samples. One-way ANOVA with Tukey test was used to determine differences of means, p values are shown above brackets.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: A,B. Correlation between anti-triS serum and NLF IgA (A) and IgG (B) antibody titers (log 10 ng/ml) of samples collected from convalescent, vaccinated and subjects with hybrid immunity. NLF triS IgA titers normalized to the levels of total IgA from the same sample. Pearson correlation coefficient and p values are shown.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: A, B. Anti-triS IgA (log 10 ng/ml) measured in NLF (A) and serum (B) of vaccinated with 2 or 3 doses, or convalescent. (C) Anti-triS s-IgA (log 10 AU/ml) antibody titers in NLF of vaccinated with 2 or 3 doses, or convalescent. D,E. Anti-triS IgG (log 10 ng/ml) measured in NLF (D) and serum (E) of vaccinated with 2 or 3 doses, or convalescent. One-way ANOVA with Tukey test was used to determine differences of means, p values are shown above brackets. The mean + 3SD of 11 negative samples was used to set a cut-off for anti-triS IgA, s-IgA and IgG in NLF. The mean + 3SD of 56 pre-pandemic serum samples was used to set a cut-off for anti-triS IgA in serum samples. The mean + 3SD of 48 pre-pandemic serum samples was used to set a cut-off for anti-triS IgG in serum samples.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: Correlation between anti-triS IgA and s-IgA antibody titers in NLF collected from convalescent, vaccinated and subjects with hybrid immunity. Pearson correlation coefficient and p values are shown.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: A, B. Anti-triS IgA (log 10 ng/ml) measured in NLF (A) and serum (B) of convalescent or subjects with hybrid immunity. (C) Anti-triS s-IgA (log 10 AU/ml) antibody titers in NLF of convalescent or subjects with hybrid immunity. D,E. Anti-triS IgG measured in NLF (D) and serum (E) of convalescent or subjects with hybrid immunity. One-way ANOVA with Tukey test was used to determine differences of means, p values are shown above brackets. The mean + 3SD of 11 negative samples was used to set a cut-off for anti-triS IgA, s-IgA and IgG in NLF. The mean + 3SD of 56 pre-pandemic serum samples was used to set a cut-off for anti-triS IgA in serum samples. The mean + 3SD of 48 pre-pandemic serum samples was used to set a cut-off for anti-triS IgG in serum samples.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: (A) Correlation between anti-triS and anti-NP s-IgA titers in NLF samples of convalescent or individuals with hybrid immunity. Pearson correlation coefficient and p values are shown. (B) Levels of anti-NP s-IgA titers of convalescent or individuals with hybrid immunity. One-way ANOVA with Tukey test was used to determine differences of means, p values are shown above brackets.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques:

Journal: medRxiv

Article Title: SARS-CoV-2 convalescence and hybrid immunity elicits mucosal immune responses

doi: 10.1101/2023.03.24.23287677

Figure Lengend Snippet: (A) Each dot represents the neutralizing titer (FRNT 50 ) of an individual NLF sample against SARS-CoV-2 ancestral (A) and Omicron BA.5 variant (B). One-way ANOVA with Tukey test was used to determine differences of means, p values are shown above brackets. Anti-triS s-IgA and IgG titers in NLF from the same subjects were correlated with FRNT 50 neutralizing titers against SARS-CoV-2 ancestral (A) and Omicron BA.5 variant (B). Pearson correlation coefficient and p values are shown.

Article Snippet: Following 3 washes with PBS/0.1% BSA/0.1% tween-20, mouse anti-Human IgA secretory component antibodies (Catalog # ABIN6155159, Antibodies online) was added to microspheres and incubated for 1 hour at room temperature.

Techniques: Variant Assay

Journal: bioRxiv

Article Title: Antibody responses to SARS-CoV-2 mRNA vaccines are detectable in saliva

doi: 10.1101/2021.03.11.434841

Figure Lengend Snippet: Purified human IgG, purified SIgA and recombinant IgA 1 lambda (see ) were coated on to ELISA plates and detected with the same goat anti-human IgA-HRP conjugate used in the assays to detect saliva and serum IgA S-protein antibodies. The plot shows net OD 450 values as a function of the amounts of the three antibodies added to the ELISA wells during the coating stage.

Article Snippet: The saliva or SIgA samples were added to wells for 1 h at room temperature, the plates were washed 3 times with PBST before addition of 50 μ l of a 1/500 dilution of Monoclonal Anti-Secretory Component IgA (Sigma I6635) that had been conjugated to HRP using the Conjugation Kit-Lighting-Link according to the manufacturer’s instructions (ab102890 HRP).

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay